Protein footprinting by pyrite shrink-wrap laminate.

نویسندگان

  • Micheal Leser
  • Jonathan Pegan
  • Mohammed El Makkaoui
  • Joerg C Schlatterer
  • Michelle Khine
  • Matt Law
  • Michael Brenowitz
چکیده

The structure of macromolecules and their complexes dictate their biological function. In "footprinting", the solvent accessibility of the residues that constitute proteins, DNA and RNA can be determined from their reactivity to an exogenous reagent such as the hydroxyl radical (·OH). While ·OH generation for protein footprinting is achieved by radiolysis, photolysis and electrochemistry, we present a simpler solution. A thin film of pyrite (cubic FeS2) nanocrystals deposited onto a shape memory polymer (commodity shrink-wrap film) generates sufficient ·OH via Fenton chemistry for oxidative footprinting analysis of proteins. We demonstrate that varying either time or H2O2 concentration yields the required ·OH dose-oxidation response relationship. A simple and scalable sample handling protocol is enabled by thermoforming the "pyrite shrink-wrap laminate" into a standard microtiter plate format. The low cost and malleability of the laminate facilitates its integration into high throughput screening and microfluidic devices.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Reliabilty-based Torsional Design of Reinforced Concrete Beams Strengthened with CFRP Laminate

 The structural reliability of torsional concrete beams strengthened with full and strip wrapping of carbon fiber reinforced polymer (CFRP) laminate is investigated. The first order-second moment reliability method has been applied to make a reliability assessment on the torsional capacity design by technical guideline in Iran. The code No.345 published by the Management and Planning Organizati...

متن کامل

Reusing (Shrink Wrap) Schemas by Modifying Concept Schemas

We provide mechanisms that facilitate database design based on a shrink wrap schema. A shrink wrap schema is a well-crafted, complete, global schema that represents an application. We develop the notion of concept schemas as a way to decompose shrink wrap schemas. A concept schema is a subset of an application schema that addresses one particular point of view in an application. To aid in shrin...

متن کامل

Sale Binary Options Safford

signals DuBois and along the actin myofilaments. Carson, Rachel. Shrink-wrap and cardboard packaging are only infrequently seen, landfills are effectively permanent facilities; that is, they will need to be monitored and maintained for Saffor after they close.

متن کامل

Volume changes occurring in postoperative below-knee residual limbs.

Comparative maturation rates of 36 below-knee postoperative, healed amputation residual limbs were observed. Measured were the limb volumes and circumferences. Three methods of residual limb stabilization were employed: 1) elastic wrap; 2) plaster cast and pylon; and, 3) plastic laminate socket and pylon. The limbs receiving the plastic laminate showed the most rapid stabilization, while the el...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Lab on a chip

دوره 15 7  شماره 

صفحات  -

تاریخ انتشار 2015